Microscopes FACS

Instrument
Zeiss Axiovert 200M
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Objectives: (our objectives require size 11/2 cover glasses).
Dry (10x)
Oil (40x, 63x, 100x)
Learn more about objective specifications from Zeiss.

Channel specifications:
1 Transmitted Light Brightfield
2 31000v2 : DAPI/Hoechst/AMCA
EX: 350/50 DM: 400LP
EM: 460/50
3 41017 : Endow GFP Bandpass Emission
EX: 470/40 DM: 495LP
EM: 560/80
4 41007a : Cy3™ (With narrow band excitation filter)
EX: 545/30 DM: 570LP
EM: 610/75
5 41008 : Cy5™
EX: 620/60 DM: 660LP
EM: 700/75

Compatible Fluorophores: (This list is not exhaustive; other fluorophores may also be compatible)
2 DAPI or Hoechst or AMCA
3 FITC or Alexa 488 or GFP or DiO Acradine Orange
4 TRITC or Cy-3 or Alexa 546 or Rhodamine
5 Cy-5

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