Microscopes FACS

Instrument
Nikon E600 Flourescence Microscope
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A flourescence microscope with a motorized stage and automatic switching between flourescence chanels. This microscope is ideal for digital capture of traditional epi-flourescence as well as for capturing 3D image stacks for deconvolution. Digital imaging is done with a 2 mega-pixel RT Slider SPOT camera from Diagnostic Instruments. Imaging of single images is done with the "SPOT advanced" software, and 3D staks are captured through "Simple PCI". The images are captured on to a Pentium III from Technical Instruments running Windows XP. All your data files must be removed from the computers hard disk at the end of your session.

Objectives:
Dry (4x,10x, 20x, 40x, )
Oil (100x)
Filtersets:
1a Ex: 330-380, Em: 435 LP
1b
2 Ex: 450-490, Em: 510-550BP
3 Ex: 530-560, Em: 590-650BP
Compatible Fluorophores:
1a DAPI
1b Cy-5
2 FITC or Alexa 488
3 TMR or Cy-3 or Alexa 546

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