Back to Instructions

Using the Micropublisher Camera

The Micropublisher Camera is a 5 mega-pixel, cooled CCD, capable of brightfield as well as fluorescence imaging. For now this camera is mounted on the Olympus Stereozoom, but in the future we will be able to use this camera on the Nikon E600 as well.

Notes:

  • The camera has a greater magnification and a smaller field of view than the oculars.
  • The camera is not quite par-focal with the oculars. Focus with the oculars first, then adjust the focus while watching the live capture on the screen.

Operation:

  1. Plug the firewire cable into the back of the camera. If this cable is in place the camera is powered and on. A diode on the front of the camera should light green.
  2. Start the computer.
  3. Log into you user account.
  4. Start Adobe Photoshop.
  5. Looking through the oculars of the microscope, set the magnification, light source and focus for your image.
  6. In Photoshop go to [File>Import>QImaging Digital Camera]
    • "QCapture Plug-In" window should open with a preview of your current image.
    • "Camera Settings" window should open.
    • If there is "No Camera Found" check to see if the fire wire cable is plugged in, and make sure there is no other software interfacing with the camera.
    • In the "Camera Settings" window has several tabs. Most use can be done in the "Basic" tab.
  7. Focus the image:
    1. Turn on binning (3 or 4) to make the camera more responsive (i.e. shorter exposure).
    2. Engage the "Auto Exposure" button.
    3. If necessary increase the gain and decrease the exposure time manually.
    4. While watching the screen, use the fine focus to slowly bring the image into focus.
    5. Set binning back to 1.
  8. Set the white balance:
    1. Use the cursor to draw a rectangle on the screen that highlights an empty/white region. (Move your sample if necessary).
    2. Click the "White Balance" button.
  9. Click the Camera icon in the tool bar to capture your image.

[top]


SFSU Link
Last modified 11/6/03.